Bead-assisted Acoustic Differential Extraction of Sperm Cells in Dilute Samples for Potential Forensic Analyses
نویسندگان
چکیده
Here we describe an improved method of separating sperm cells from lysed epithelial cells via acoustic cell trapping. In particular, we show that in the processing of samples with low numbers of sperm cells, addition of polymeric beads to the sample[1] allowed for better performance in terms of higher trapping efficiency versus our previous acoustic differential extraction (ADE) results[2]. We report an increased throughput of 30 μL/min (1.8 mL/hour) and reduced sample concentration requirement (1 sperm cell/μL), Separation of low sperm count samples in epithelial cell lysate allowed unambiguous STR typing of the sperm donor.
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